Deliverables WP4
4.1 |
Optimised protocol for proteomics analysis (SILAC) of NSC34 cells, iPSCs or definition of a SILAC standard from other cells that represent the proteome of iPSCs |
18 |
4.2 |
Devise protocol for streamlined SNP pull-down |
24 |
4.2.1 |
SNP pull-downs for the SNPs in the ALS associated genomics regions |
44 |
4.2.2 |
50 SNP pull-downs |
48 |
4.3 |
Proteomic data on iPSC-derived MNs |
30 |
4.4 |
Proteomic determination ALS aggregates |
30 |
4.5 |
Quantitative interaction data on the candidate ALS proteins in NSC34 |
30 |
4.6 |
25 quantitative protein-protein interaction assays) |
48 |
4.7 |
Proteomic comparison of differentiated (diseased) iPS derived motor neurons with undifferentiated (healthy) iPS controls |
48 |