4.1

Optimised protocol for proteomics analysis (SILAC) of NSC34 cells,  iPSCs or definition of a SILAC standard from other cells that represent the proteome of iPSCs

18

4.2

Devise protocol for streamlined SNP pull-down

24

4.2.1

SNP pull-downs for the SNPs in the ALS associated genomics regions

44

4.2.2

50 SNP pull-downs

48

4.3

Proteomic data on iPSC-derived MNs

30

4.4

Proteomic determination ALS aggregates

30

4.5

Quantitative interaction data on the candidate ALS proteins in NSC34

30

4.6

25 quantitative protein-protein interaction assays)

48

4.7

Proteomic  comparison of differentiated (diseased) iPS derived motor neurons with undifferentiated (healthy) iPS controls

48